SERVICES

Through the aid of its Facilities and Equipments, COMT offers to the scientific community, to public and private institutions and to private companies a number of Laboratory Services that may contribute to the advancement of the customers’ research and product development efforts. Provided Services rely upon basic biochemical, cell biological and molecular biological methods, or more advanced techniques and protocols that, in part, have been developed by core Units of the Centre. Thus, the custom Services that can be requested may be based upon established and standardized procedures, propriety methods and technologies, or may be tailored to satisfy the customer’s needs.

The modalities and costs involved in the performance of the Services, and the delivery of their output, are accurately defined for each Service. For requesting a quotation, customers are invited to fill out the “Service Request” form found on this web page. Please note that in the price listing "Internal price" and "External price" refer to the price charged to Internal or External users respectively, as defined by the Centre’s Regulations. These prices may be negotiated with the indicated Reference persons, or with the Director of the Centre. Naturally, any requested Service is bound by strict confidentiality and is performed in accordance to the ethical rules of the Centre.

Investigators are also welcome to contact COMT for advice and problem solving, in relation to specific conceptual, methodological or technical aspects pertaining to the Services offered by the Centre. Such “consulting assistance” will be charged separately from the list prices detailed below and is subject to specific negotiation.

PRODUCTION, CHARACTERIZATION AND LABELING OF POLYCLONAL AND MONOCLONAL ANTIBODIES AND IMMUNOCHEMICAL SERVICES

Production, purification and characterization of mouse monoclonal antibodies Service 1 Conventional immunization protocol on Balb/c mice using a costumer-supplied antigen (immunogen). We usually use 3 female mice per immunization, but it is possible to increase the number of animals if deemed necessary. The minimum amount of antigen for the completion of phase 1 and phase 2 of this procedure is 1 mg. Intraperitoneal antigen injection is followed by spleen extirpation and fusion of mouse spleen cells with myeloma cells. Antigen-reactive…

ANALYTICAL AND FUNCTIONAL PROTEOMICS

Production of recombinant proteins Service 1 Production of recombinant proteins in E. coli, yeast cells, specifically engineered human HEK293 or Chinese hamster cells CHO cells, starting from appropriate plasmids (viral or not) provided by the customer and containing the specific tag-fused cDNA as ready-to-use reagents for transfection into the desired model cells. References Dr Ileana Ramazzina, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., Tel. (+39) 0521-903790 Dr Serena Galati, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., Tel. (+39) 0521-905608 For the prokaryotic expression system: Internal price - 1.250 Euro/1 milligram…

FUNCTIONAL GENOMICS AND POST-GENOMICS

Cloning and vector production for transfection of prokaryotic and eukaryotic cells Cloning into transfection plasmids, with or without reporter genes is offered starting from cDNAs and plasmids provided by the customer or by accessing cDNAs and plasmids provided by COMT. This service includes the production of mutated constructs by site-directed mutagenesis of amino acid residues, constructs carrying specific deletions or constructs containing fragments of the cDNA of the gene of interest. References Dr Silvia Rossi, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., Tel. (+39) 0521-905655…

ULTRASTRUCTURAL ANALYSES

Transmission Electron Microscopy (TEM) References Prof. Prisco Mirandola, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., Tel. (+39) 0521-033052 I) Sample preparation Internal price - 125 Euro/sample External price - 225 Euro/sample II) Sample processing and analysis Internal price - 550 Euro/sample External price - 950 Euro/sample Scanning Electron Microscopy (SEM) References Prof. Prisco Mirandola, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., Tel. (+39) 0521-033052 I) Sample preparation Internal price - 125 Euro/sample External price - 225 Euro/sample II) Sample processing and analysis Internal price - 250 Euro/sample External price -…

CELLULAR ASSAYS IN VITRO

Conventional and custom-designed in vitro cellular assays, based upon flow cytometry, real-time microscopy, laser scanning cytometry, parallel-flow assays, cell sorting, impedence measurements and multi-parametric fluorescent platforms, are offered for the analysis of cell proliferation, cytotoxicity/apoptosis, signal transduction, cell adhesion and cell migration/invasion Service 1 – Flow cytometry Qualified flow cytometric analyses are offered on cells supplied by the customer or available at COMT. Antibodies used for the analysis can be either provided by customer or by COMT. Prices listed below…
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